I got my PhD in Physical Sciences in Paris and worked at that time on nucleic acid-protein complexes using spectroscopic method. I then focused my research activity on synthetic oligonucleotides for artificially regulating gene expression, notably in viruses (HIV, HCV) and protozoan parasites (trypanosome, leishmania). Presently, at the head of a group working on oligonucleotide aptamers, I am interested in the design of artificial riboswitches for regulating, probing, sensing in the perspective of developing devices of biomedical interest.
About our lab
Our group (University of Bordeaux) is part of a large research unit (ARNA = “RNA: natural and artificial regulation”) affiliated to “Inserm”, the French organism for Medical Research.
The unit brings together multidisciplinary expertises on nucleic acids: cell and molecular biology, structural biology, nucleic acid chemistry and biotechnology. We have access to state of the art facilities for cell culture, structural investigation (NMR, X-ray crystallography, mass spectrometry) and organic synthesis. The Unit has a very international character and hosts many PhD students and post-docs. We develop collaborations with foreign and French teams. Our team is strongly connected to Novaptech, a spin off that offers to design aptamer-based tools.
About the project
Project 2: We have recently described aptamer-based biosensors taking advantage of loop-loop (kissing) interactions (Durand et al., Angewandte Chemie, 2014). We will make use of this technology for tayloring sensors able to respond to the presence of tumor markers in live cells, signaling their presence by a signal (fluorescence, …) The project will require the use of SELEX and biophysical methods (SPR, fluorescence anisotropy, …) for characterizing the sensor-ligand complexes.